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Successfully prevent infection of cultured cells by a Herpes Simplex virus (HSV-1)

sans

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sans

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Paris, January 19, 2011 – Cellectis (Alternext: ALCLS), announced today that scientists from Cellectis therapeutics, the French National Center For Medical Research (CNRS) and Institut de la Vision (Paris) have used its proprietary Meganucleases to successfully prevent infection of cultured cells by a Herpes Simplex virus (HSV-1). The research has just been published online by Molecular Therapy (Mol. Ther. 2011, Jan 11. Epublication ahead of print). These are the first proof-of-concept data showing that Meganucleases can prevent viral infection. In this article, scientists used HSV-1 specific Meganucleases engineered by Cellectis to successfully prevent the infection of human cultured cells by HSV-1. Subsequent analysis of the treated cells using deep sequencing techniques showed that inhibition of HSV-1 infection by the anti-HSV-1 Meganucleases was associated with cleavage of viral DNA (or virus clipping). When the virus is clipped at a well-chosen and specific location, this prevents its normal activity as well as its ability to replicate and spread.

“Virus clipping using Meganucleases is a new therapeutic approach to tackle persistent viral infection which could have broad applications in medicine”, said Pr. José Alain Sahel, Director of Institut de la Vision. “We are particularly interested in the potential of Meganucleases in the prevention of recurrent infection during corneal transplantation and look forward to continuing our studies in this area.”

"The results published in Molecular Therapy are very exciting since they open new fields of research for fighting HSV recurrent infections, a major cause of both acquired and severe loss of vision", added Dr. Marc Labetoulle, M.D., Ph.D. of CNRS.

About HSV-1 infection HSV-1 infection is highly prevalent globally, with an estimated 70-90% of the adult population carrying the virus. The majority of seropositive individuals are unaware that they are infected due to the asymptomatic nature of their infection. But once infected, the virus remains in the body for life. HSV-1 accounts for 20 to 40% of genital herpes infections and 95% of oral, neural or ocular herpes infections. It is a leading cause of blindness throughout the world and remains a major cause of corneal graft failure. A cure for herpes has yet to be developed.

http://www.cellectis.com/media/press-release/2011/cellectis%E2%80%99-meganuclease-technology-used-efficiently-prevent-viral-infectio-0

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PeacefulClarity Honeycomb Member

PeacefulClarity

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Hi everybody , i was told that i had herpes type 1 and type 7 and that type seven manifests as occasional flarings up on the skin called Pityriasis Rosea (but that a person can develop Pityriasis Rosea without ever having herpes). When i looked up typed 7 online i didn't find much information. Has any body ever experienced Pityriasis Rosea as a result of having herpes?.

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struggle83 Acknowledged Support Member

struggle83

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Hi everybody , i was told that i had herpes type 1 and type 7 and that type seven manifests as occasional flarings up on the skin called Pityriasis Rosea (but that a person can develop Pityriasis Rosea without ever having herpes). When i looked up typed 7 online i didn't find much information. Has any body ever experienced Pityriasis Rosea as a result of having herpes?.

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    • sgt98
      What is the usual order of events in initial infection for GHSV?

      By sgt98 · Posted

      Hey @WilsoInAus ok thank you, I will do my best to move on and stop trawling forums haha. 
    • WilsoInAus
      What is the usual order of events in initial infection for GHSV?

      By WilsoInAus · Posted

      Hey @sgt98 but there is no feasible infection or outbreak to suppress and antivirals do not suppress an initial outbreak in any event 
    • sgt98
      What is the usual order of events in initial infection for GHSV?

      By sgt98 · Posted

      Thanks @WilsoInAus I understand apologies for this but the only other thing I am worried about is that I did take a course of Famvir on day 1 as I felt like I was having an outbreak of cold sores and am worried that has suppressed the initial outbreak genitally 
    • WilsoInAus
      What is the usual order of events in initial infection for GHSV?

      By WilsoInAus · Posted

      Hi @sgt98 it is not feasible to obtain a HSV-2 infection from receiving oral sex - only HSV-1 is feasible but you've already got that and immunity from any further infection with HSV-1. You do not need any further tests for HSV. You're feeling regret, try to forgive yourself and calm down, let the rational take over. You know the answer here and it won't be long until you believe it too.
    • WilsoInAus
      Looking for some insight on an atypical experience.

      By WilsoInAus · Posted

      Hi @NerdP423 and welcome to the website. You raise a lot of points and I think the best way to address your concerns is add some comments at each key point. I've copied your note again below and added these comments in square brackets. I appreciate any insight (even speculation) as to what has been going on with me. I'm a 37y/o M. I last was intimate with a new partner on 2/11/2022, and a few days later started having a huge amount of discomfort in my face. [The first thing that happens though is that herpes causes lesions or at least some skin based disruption. Other symptoms are then related to the actions of the immune system responding to the virus. Without lesions, it is highly questionable that the ailment is related to herpes, yet testing is useful if you have concerns as you have done so.] About a month after that [herpes causes issues within days, if the first 'symptoms' are a month later - its extremely unlikely they are related to herpes], significant discomfort downstairs, however every test I have ever taken for HSV 1 and 2 has been negative. Here are the details: Face: Previously, some significant tingling and itching on the right side of my mouth, lips and chin (still there, but milder) [herpes does not cause general tingling and itching, it can cause a reasonably concentrated feeling of itch/throb from which a herpes lesion appears within hours]. Occasional hot flashes near my right eye, cheek and ear [herpes does not do this, it may be a immune response to something, or stress]. Sometimes it will feel like the skin is crawling on the right side of my face [herpes does not cause a general crawling sensation]. Never seen anything that looked like a traditional cold sore [that's extremely telling, even people with associated atypical symptoms will have experienced herpes lesions]. Occasionally, the left side of my face will have a momentary feeling of skin crawling, but it's so mild that I am not really worried about it. Downstairs: Thankfully, most of these are now milder than they were before. Occasional momentary pinch of pain at the base of my genitals. [herpes does not cause a general pinch feeling.] Aching pain in my boxer area (groin, leg folds) [nor this] Occasional feeling of cold in my boxer area, butt, or lower back. ( also in my shins and occasionally even my arms) [nor this] On 4/30/2022, I had been in discomfort for almost two months. I scratched an itch, noticed it hurt, and then checked - I did have an open ulcer down there. Took myself to the ER to get swabbed, came back negative. [If this was a PCR then this will be very conclusive.] Practitioner said it may come back negative because it was already open. However the lab report noted that it definitely didn't look like what you'd expect a typical first herpes outbreak to look like [how would the lab know??]. All blood tests negative so far. I had two western blots, the second one was nine months after exposure. [Two negative Westernblots!!! Many that's real convincing]. All other swabs also negative. I went to urgent care, because I know the timeliness of when the Swab is taken matters. One time, a swab was not done, because practitioner said it was folliculitis and wouldn't swab it (It was at my belt line). [Belt line is highly unlikely to relate to herpes.]   A blood test revealed I had low-ish B12 (technically in range, but at the very low end, especially for a man of my size). I had a series of B12 injections, and I am taking a B12 supplement. I am not taking any lysine or arginine at the moment. I was taking the Arganine to see if I could induce an outbreak. [This is irrelevant, there is no known linkage between herpes outbreaks and arginine/lysine intake - its a myth - and B12 infers nothing.] I think what I am asking is - has anyone here ever repeatedly tested negative over and over again over long period of time, before getting a definitive answer, be a positive test, or something else? [The answer to this is: Extremely few people with a HSV-1 infection and even more rarely HSV-2 test repeatedly negative on Westernblot and actually carry the virus. Of the cases that I know of that had delayed detection by a swab and negative blood tests in the meantime (and that's only 3-4 cases), they had some form of lesions within days of infection but did not obtain a swab for various reasons and then obtained a positive swab of a subsequent lesion with the record being 11 months later. A couple of the cases did have some 'background' symptoms they thought might be related to herpes but that isn't ascertained and some did not have any unusual symptoms at all apart from the lesions. Hence as you did not have lesions around your mouth or lips within days of the last sexual encounter as is exceptionally common for a primary oral HSV-1 infection, that pretty much rules out herpes orally as it is. The fact that you had no genital symptoms for a month also rules out genital herpes. I am not aware of anyone at all who has genuinely gone on to test positive by swab or blood in your specific circumstances. There are hundreds if not more than a thousand experiences on this website alone that are similar to yours that are truly negative for herpes I'm one of them!]  
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