Go beyond the abstract,
JVI Accepted Manuscript Posted Online 22 June 2016
What are they doing and why?
344 Studies of the mechanisms silencing viral lytic gene
345 expression will require in vivo animal models, in which it is difficult or impossible to
346 perform techniques such as transfections or knockdowns. The primary aim of the
347 experiments described here was to develop a method of performing these experiments
348 in vivo.
The self-complementary (scAAV) genomes they generated cut in half the transgene carrying capacity of the vectors from 4.5 kb. This study is about developing a means to delivery GFP genes, or some ORF for small RNA so as to study a latent infection. (Lines 84-87) . Note I recall Duke already said 4.5 kb limited them. This study needed to go even smaller. Basically all they can do right now is just send in GFP genes.
378 studies using AAV to deliver shRNA or to express other gene products such as
379 signaling pathway agonists or chromatin modifiers will allow us to test this methodology
380 in the relevant in vivo models, targeting genes or proteins in neurons that are co-
381 localized with the AAV vector, rather than globally inhibiting or up-regulating gene
line 349-350 "Results of IHC experiments demonstrate that, AAV vectors transduce DRG sensory neurons". The vectors don't go everywhere HSV replicates (nerve fibers, neighboring cells, glial cells, motor neurons, etc.) Albeit AVV8 did not have a neural preference to only sensory neurons.
Bottom line this stuff is in its infancy. I wish someone would focus on adding shRNA targeting HSV-2 into these vectors... but we need to wait and see...
Thank you @gonnakillher for standing up for me when I didn't stand up for myself. I regretted not saying more, I just apologized on the spot because I don't like to make people feel bad.
But blaming victims of the stigma - whether diagnosed yet or not - for the stigma, is ridiculous. Especially me, who knowingly engaged in sex with a woman who told me she had HSV-2. I read a lot about it, learned how common and under diagnosed it is, and how low risk a single protected exposure is, or even several of them. And even if I miraculously don't have it - if I found another woman with this virus, at this stage in my life, I'd give her a chance, too - I'd likely just wait longer to engage.
Because I'm terrified of the potential stigma does not make me a bad person, neither does discussing it online. Where else can I talk about how I feel? With friends that think I'm crazy for not believing 14 week test results? If I have to worry about expressing my feelings here, in a discussion I started, you really have diminished the value of this forum for me.
Even joining the dating site had tremendous value to me on a particularly depressing Tuesday evening. Just knowing that if these textbook phn pains are in fact phn, there are women just like me out there.
i haven't read the success stories but I'll check them out. I have read stories of people like us who don't test positive and eventually learn that they are in fact negative. It's possible. If it were up to @Free73 Id only hold out hope for that sort of outcome - not letting myself contemplate and brace for the other outcome. Silly.
Its only made for research purposes in quantities so small that its incredibly expensive (~$1/1 mcg). The dose predicted to work the best is 150 mcg, thus basically right now its $150/day to use it.
Amenamevir is what the Japanese are focusing on and its also effective against Chickenpox, whereas Pritelivir is not. Not sure if the Japanese story is true, but again, its very expensive if they got it.
OK you wise herpes gurus on here, I had this thought and I want your "expert" opinions.
I'm OBSESSED with the fear of transmitting. I know not to have sex during OB. I plan on ALWAYS using condoms and I am on daily Valtrex. I know there is still a 2% chance of spreading. I want to decrease the odds even more.
So... I thought about this skin barrier spray. If I spray it over the boxer short area before sex, I'll wonder if it would keep asymptomatic shedding virus to myself and away from my partner. Thoughts???
Edit- spraying on MY skin, not partner's