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alright, I'm gonna calm down but this is exciting info.   Apologies, I'm a bit too impatient to wait for Cullen and Bloom's results on rats, so I've decided to scratch my itch for wondering if CRISPR will get into the nervous system by looking elsewhere.    ...My heart goes out to all the lab animals... I never thought I'd be in a position where I'd want to see what happens to another life before I get excited about it but here I am... I know, here in the US and other places, they take care in being humane with "sacrificing" lab animals.  Not trying to play on stereotypes but I hope the animals in China are treated with the same respect.


Pig shows multiple superior characteristics in anatomy, physiology, and genome that have made this species to be more suitable models for human diseases, especially for neurodegenerative diseases, because they have similar cerebral convolutions compared with human neocortex. Recently, CRISPR/Cas9 system shows enormous potential for engineering the pig genome. In this study, we expect to generate human Parkinson's disease pig model using CRISPR/Cas9 system by simultaneously targeting three distinct genomic loci, parkin/DJ-1/PINK1, in Bama miniature pigs. By co-injection of Cas9 mRNA and multiplexing single guide RNAs (sgRNAs) targeting parkin, DJ-1, and PINK1 genes, respectively, into in vivo derived pronuclear embryos, we simultaneously targeted three distinct genomic loci. The gene modified piglets remain healthy and display normal behavior at the age of 10 months. In addition, despite the high number of sgRNAs were employed in the present study, our trio-based whole-genome sequencing analysis suggested that the incidence of off-target events is low. Our results demonstrate that the simplicity, efficiency, and power of the CRISPR/Cas9 system to allow for the modification of multiple genes in pigs and yield results of high medical value.


Author information

  • 1State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
  • 2University of Chinese Academy of Sciences, Beijing 100049, China.
  • 3State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
  • 4Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • those chinese and their love of pork...
Edited by Sanguine108
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That is really good news! Parkinson's disease is no joke. A neurodegenerative condition. So I would think treatment for Parkinson's would be a lot more difficult than treatment for HSV. Since these test pigs are still healthy. It's a great sign them Cullen mice will be as well.


Edited by dont quit!
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