CHINESE SCIENTISTS SAY THEY’VE FOUND A SAFER ALTERNATIVE TO CRISPR
Researchers from China’s Peking University have developed a new gene-editing technology — and they think it shows promise as a CRISPR alternative for fighting human diseases.
According to a paper published on Monday in the journal Nature Biotechnology, this new technology, LEAPER, which stands for “leveraging endogenous ADAR for programmable editing of RNA,” works similarly to CRISPR-Cas13, targeting RNA molecules as opposed to DNA like the well-known CRISPR-Cas9.
But while CRISPR-Cas13 relies on both a guide RNA and the Cas13 enzyme to make its edits to RNA, the LEAPER system needs just one component known as an arRNA. This makes the system “more easily deliverable and less likely to result in unwanted cellular immune responses,” the researchers told China-based media outlet Caixin.
As for the potential uses of their CRISPR alternative, Peking researcher Zhou Zhuo told Caixin that “there are clear prospects for using this technology in disease treatment.”
In fact, in tests of cells taken from people with the debilitating genetic disorder Hurler syndrome, LEAPER was able to correct “sufficient amounts” of the cells’ mutated RNA, University of Queensland geneticist Ernst Wolvetang, who wasn’t involved in the research, told Caixin.
However, the research is still in the early stages and is only now moving into tests on animals — meaning we won’t know for some time if LEAPER will dethrone CRISPR as the preeminent gene-editing technology.
READ MORE: Chinese researchers create safe gene-editing tech that avoids ethical concerns [The Global Times]
Hi everyone, I create a new topic for all herpes cures as I find it difficult to navigate to different topics to get news from different therapies so if anyone has any news, please feel free to post here to keep everyone updated, optimistic and focusing on making the cure available faster.
According to my understanding (please correct me if I'm wrong), the herpes virus enters our bodies first then invades and replicates then retreats to hide in our neurons. Our immune system could fight the virus itself but can't chase it up to nervous system and can't detect it once it goes dormant. Immune system is an amazing soldier because any outbreak couldn't last for more than 14 days or so (maybe less) since our immune system always keep our body in check and once an outbreak happens, it will find the replicates of the virus then destroy them which is when the outbreaks go away so if we can destroy the latent virus, we can 'cure' the disease.
Here are all the potential cures I know, some are just vaccines and not cure but I believe it would help fight the virus:
1. Dr. Keith Jerome (Fred Hutch Center): (credit to @MikeHerp on GREAT NEWS! Dr. Jerome at Fred Hutch has achieved 50% to 90% elimination of latent HSV in mice)
Category: cure 😆 Method: Injection Strategy: Using the gene editing with meganucleases to act as "molecular scissors", go into the infected nerve cells and either clip out parts of the virus, or basically destroy the entire thing. Clinical trials time: none. Still in pre-clinic but very promising and Dr. Jerome said he believed there would be a cure in his lifetime. (I tried googling his age but couldn't find anything lol) Target: HSV1&2, I believe. Jerome has been able to achieve 50% to 90% elimination of latent HSV in mice, depending on the location. In vivo. I mean elimination, as in, the latent virus DNA is fragmented and dissipates--not just edited to render it inactive. Completely GONE from the neuron. Elimination of 90% of latent HSV from Sacral ganglia resulted in decrease in viral replication by over 90%. Elimination of 50%+ from trigeminal ganglia in mice, resulted in 50% reduction in viral replication. They have set out a pathway to increase HSV latent elimination beyond 90%. This is all with just ONE treatment. Keep in mind that they've gone from 2-4% editing of latent virus in mice in 2016, to elimination of up to 90% in some ganglia in just 2.5 years. Stunning progress. Currently either perfecting the cure to remove 99.9% of the virus in mice or testing on guinea pigs. 2. CRISPR-Cas9 (ExcisionBio): I do not know much about this project so please put in some info if you have
Category: cure 😆 Method: Injection Strategy: locate the DNA sequences and delete the whole stretches and use a different protein which is larger than meganucleases of Dr. Jerome. Clinical trials time: 2021. They have some trials going on but for HIV and cancer. Target: HSV1&2 They made a solid foundation for concept of curing more complex human disease with gene therapy is absolutely possible. The problem with this method is controlling how much of the DNA the method will delete and the cure particle is too big to reach to the virus cell in neurons. However, from this article, it shows that Fred Hutch Center (FHC) had found a way to carry the gene-editing therapy to virus cell by using gold nanoparticle instead of inactive virus cell. They made the gold nanoparticle big and sticky enough to hold the gene-editing material but also small enough for the cell to absorb. They used Cas12a instead of Cas9 cuz it makes a staggered cut in DNA and it only requires one molecular guide while Cas9 needs two. This method has no toxic effects so far. This article is for HIV research but the gene-editing for HSV has delivery problem and this solves it, plus, herpes virus is localized and it's not all over the body like HIV so I believe it would be easier and effective to use this method for herpes cure.
Note: if you find new cure, medicine or vaccine, please follow the same format.
1. Vaccine/medicine/cure name (Company)
Category: cure/suppressed therapy/therapeutic vaccine/preventative vaccine Method: injection/oral medicine/skin cream/etc Strategy: (how they are going to deal with the virus) Clinical trials time Target: HSV1/HSV2/HSV1&2/Without HSV Other details
Hey guys thought id share this article with you all.
By Cpt. Hindsight
So I’m certainly no doctor/scientist, but this new CRISPR tool sounds pretty promising. Towards the end of the article, they talk about how the new CasRx was able to penetrate the neurons in their in vitro experiments with an 80% success rate for treating FTD. Neat stuff!