HSV CRISPR Research coming out of China

[Unrequited]

So China are trailblazing Crispr technology, and HSV is part of that, interesting very recent articles.

 

https://www.ncbi.nlm.nih.gov/pubmed/29691470

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795453/

https://hsvupdate.blogspot.com/

I just want to add... and I've seen this plenty of times, but having read it again recently. I am quite confident about it.

The Editas Lifesciences Information  - Clinical trials 2019-2020!!!!!

HSV-1/2 CRISPR Cas9/CasY/gRNA ICP Triplex biologic (EBT104) 

We have developed a CRISPR biologic for the targeting of an HSV capsid protein which is necessary for virion maturation (EBT104). We have conclusively shown and verified in peer-reviewed publications that disruption of this viral gene with EBT104 impedes HSV replication.

We are currently in animal studies utilizing nanoparticles capable of cross the blood-brain barrier to deliver this potent therapeutic that disrupts the HSV replication cycle.

So..... as it says here firstly, the have "conclusively shown and verified" that their treatment disrupts HSV replication, and are using nano-particles that cross the blood brain barrier where latent virus is stored. It is a "therapeutic". As such, it's a therapeutic which well, sounds to me, could easily become a functional cure.  As they term it a "knockout" which means in Crispr terminology, removal of parts that make it dangerous, or spread. 100% knockout means, 100% disruption. And, what's more, they are testing next year in live humans. Amazing!

https://www.fasebj.org/doi/pdf/10.1096/fj.201701129R

Information on "knockout"
Excision BioTherapeutics-The company plans to start trials for both HSV-1 and HSV-2 around 2019 or 2020 depending on funding and IND status. They use a type of gene edit called "Triplex gRNA Excision Knockout" using saCas9 for HSV-1 and CasY for HSV-2. This may perhaps be the most promising therapy or functional cure to date if they're able to overcome all of the safety concerns.
Excision refers to their HSV-1 program as EBT104 and their HSV-2 program as EBT105.

[Nietzsche]

CRISPR gave us many new possibilities and great its research from China, this will pressure US HSV CRISPR research to speed up. 

[Piyush]

@Quest

[undertsun]

"We are currently in animal studies utilizing nanoparticles capable of cross the blood-brain barrier to deliver this potent therapeutic that disrupts the HSV replication cycle."

This sounds nice! Does anyone know what is the delivery mode for this approach? Is it local injection?

Edited June 10, 2018 by undertsun

[Ashia]

1 hour ago, undertsun said:

"We are currently in animal studies utilizing nanoparticles capable of cross the blood-brain barrier to deliver this potent therapeutic that disrupts the HSV replication cycle."

This sounds nice! Does anyone know what is the delivery mode for this approach? Is it local injection?

AAV delivery for both HSV-1/2. Clinical trials begin 2019-2020.

[HerpesDumbAf]

I really hope this is real and it’ll actually happen , so you think it’s possible to sign up for the trials ? 

[Goku]

Can anyone explain me what is crsipr.will it cure hsv completely.

[HerpesDumbAf]

9 minutes ago, Goku said:

Can anyone explain me what is crsipr.will it cure hsv completely.

Only a little. CRISPR is suppose to be gene editing the DNA which is suppose to work like scissors (enzyme) to cut the DNA that needs to be out and be modified so if CRISPR works then it will cure it but anyone correct me if I’m wrong I’m only going by the research I did 

[Goku]

5 minutes ago, Savannah069 said:

Only a little. CRISPR is suppose to be gene editing the DNA which is suppose to work like scissors (enzyme) to cut the DNA that needs to be out and be modified so if CRISPR works then it will cure it but anyone correct me if I’m wrong I’m only going by the research I did 

As per my understanding, current antiviral also kill the virus in blood.but only problem is the virus is hiding in nervous system.Can CRSIPR  kill the virus hiding in nervous system?

[Atrapasueños]

El virus no se encuentra en la sangre El virus no se encuentra en la sangre El virus no se encuentra en la sangre cuando se contagia una persona El virus no se encuentra en la sangre cuando se contagia una persona El virus no se encuentra en la sangre más células de la piel una vez que el sistema inmunológico genera los virus comienza a eliminar el virus, pero el virus es tan inteligente que evade el sistema inmunológico y rápidamente viaja por los axones de las neuronas y queda en el estado latente y de ahí replica y vuelve a viajar por los axones y vuelve a replicar en las células de la piel y el resultado todos lo conocemos como ampollas 

Crispr es el mecanismo que tienen las bacterias para defenderse de los virus, pero los científicos descubrieron que se puede utilizar el sistema inmunológico de las bacterias (crispr) para modificar células humanas, de animales,de virus, etc  

El problema es llegar a todos los virus del hsv y lo más importante llegar a los virus latentes (neuronas) y modificarlos para que no se repliquen 

___________________

The virus is not found in the blood hsv replicates in skin cells when a person is infected the virus penetrates the cells and takes their machinery to replicate once the replication ends the cell explodes and more viruses are released to infect more cells of the skin once the immune system generates antibodies begins to eliminate the virus, but the hsv is so smart that it evades the immune system and quickly travels through the axons of the neurons and remains in a dormant state and hence never leaves replicates and travels back through the axons and returns to replicate in the skin cells and the result we all know as blisters

Crispr is the mechanism that bacteria have to defend themselves against viruses, but scientists discovered that they can use the immune system of bacteria (crispr) to modify human cells, animals, viruses, etc.

The problem is to reach all hsv viruses and most importantly to reach the latent viruses (neurons) and modify them so they do not replicate

[HerpesDumbAf]

8 hours ago, Goku said:

As per my understanding, current antiviral also kill the virus in blood.but only problem is the virus is hiding in nervous system.Can CRSIPR  kill the virus hiding in nervous system?

It doesn’t kill it. It suppresses the virus. And the virus is found in blood but they say it takes a while  for the antibodies to show that’s why it’s false negs n positives in blood results sometimes. And they say not to say kill for a virus since it’s non living. CRISPR’s goal in this is to remove HSV1/2 completely from DNA and modify it. 

[Goku]

4 minutes ago, Savannah069 said:

It doesn’t kill it. It suppresses the virus. And the virus is found in blood but they say it takes a while  for the antibodies to show that’s why it’s false negs n positives in blood results sometimes. And they say not to say kill for a virus since it’s non living. CRISPR’s goal in this is to remove HSV1/2 completely from DNA and modify it. 

So if CRISPR succeded,then it can be able to completely cure the disease?

Let's hope it will success.

[35hope]

what i been reading from the web is the company excision will be our quickest hope for a functional cure with this virus if everything goes perfect it can be prove to be a cure with in three years from when the trials begin and hopefully in market soon afterwards

[HerpesDumbAf]

1 hour ago, 35hope said:

what i been reading from the web is the company excision will be our quickest hope for a functional cure with this virus if everything goes perfect it can be prove to be a cure with in three years from when the trials begin and hopefully in market soon afterwards

So 3 years supposed to be human trials ? This shit takin to long like to I gotta go to the labs myself lmao I kno it’s suppose to take a while but ion wanna be 30 then a cure comes loll

[IamPositive]

Whoa never heard about it, currently I’m in china tho.. well.. china has so many local/their own vaccines as far that I know. Like MenAC for infant, 4in1, influenza etc etc.. they don’t really relaying on big pharmaceutical companies.. I’ll find this info..

[SnoopCock]

Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections

http://www.mdpi.com/1999-4915/10/6/291/pdf

College of Life Sciences and Technology, Jinan University, Guangzhou 510632, China;
yuchuan1022@gmail.com

[Unrequited]

On 6/13/2018 at 9:12 PM, kill.hsv(not Kenny) said:

Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections

http://www.mdpi.com/1999-4915/10/6/291/pdf

College of Life Sciences and Technology, Jinan University, Guangzhou 510632, China;
yuchuan1022@gmail.com

Just note, the authors are not undertaking a study. This is just a literature review. Do not contact them.

[Heal World]

On 6/11/2018 at 12:49 AM, Nietzsche said:

CRISPR为我们提供了许多新的可能性和来自中国的大量研究，这将加速美国HSV CRISPR研究的速度。 

 

On 6/14/2018 at 2:12 AM, IamPositive said:

哇从来没有听说过，目前我在中国寿.. ..中国有很多本地/自己的疫苗，据我所知。像MenAC的婴儿，4in1，流感等等。他们并不真正传播大制药公司..我会找到这个信息..

 

On 6/14/2018 at 4:12 AM, kill.hsv(not Kenny) said:

CRISPR / Cas9系统对疱疹病毒感染的 潜在应用

http://www.mdpi.com/1999-4915/10/6/291/pdf

暨南大学生命科学与技术学院，广州510632;
yuchuan1022@gmail.com

It is unfortunate to tell you that graduate students or doctoral students in China must publish papers every year. These items you read are all written by Chinese medical personnel in order to accomplish their tasks. No substantive research has been conducted. Once a Chinese doctor once said that China has a large population, hsv will not cause death, so they will not have any medical research, just academic.

[veagle]

2 years in, any updates?

[StayStrongOk]

@veagle, a lot of news about CRISPR now worldwide. Browse through 'Herpes Cure Research' in this forum and you can find a bunch of info. Even recently we have first CRISPR applied to a human. Exciting! Let's keep hoping for more and more successful trials/researches.

[RNY18]

Corona Virus dominates the news...

 

[Cas9]

8 hours ago, StayStrongOk said:

@veagle, a lot of news about CRISPR now worldwide. Browse through 'Herpes Cure Research' in this forum and you can find a bunch of info. Even recently we have first CRISPR applied to a human. Exciting! Let's keep hoping for more and more successful trials/researches.

Do you know about the work being done by Dr. Jerome?

[StayStrongOk]

17 hours ago, RNY18 said:

Corona Virus dominates the news...

 

Because Covid-19 is airborne making it spread easily and has global >3% fatality rate currently. No working vaccine for now. It is becoming an epidemic (or is it already is?), It makes very good sense that the virus makes the headlines.

[StayStrongOk]

16 hours ago, Cas9 said:

Do you know about the work being done by Dr. Jerome?

How many times you want to ask me my friend? (no problem about that :))

Yes I do.

[Cas9]

13 hours ago, StayStrongOk said:

How many times you want to ask me my friend? (no problem about that :))

Yes I do.

LOL; Sorry, didn't realize it was you I had asked this question before.

OK, so the reason I asked was because Dr.Jerome's work reveals that CRISPR doesn't work when it comes to eliminating the latent virus. That's because the Cas enzymes are too large to get at the viral DNA when the virus is dormant. They are also more difficult to package into an AAV for delivery to the neurons. At least that's how I understood his video. That  doesn't mean it wont work at stopping viral replication however; and I think that's what Excision Biotherapeutics is working on; i.e. stopping replication (but the latent virus remains).

Doctor Jerome's approach is to create a homegrown gene editor that is much smaller than the CRISPR Cas enzymes. It's more difficult to make, but once you've created it, you have it for good. And he has in fact created it, so that work is done. His gene editor, which he refers to as a meganuclease, is successful at destroying the latent virus. He's achieved 50% and 90% elimination rates of the latent virus, depending on the relevant ganglion.

The issue causing him to not achieve higher rates, especially with the ganglion where he achieved 50%, is due to delivery of the meganuclease. As stated earlier, he's using the traditional delivery method (i.e. AAV vectors). There are many AAVs, even some synthetic ones. The AAVs vary on how good they are at transporting the meganuclease to a particular nerve bundle/ganglion.

So Dr. Jerome is working on finding the best AAV for each ganglion so that almost all the neurons of that ganglion, receive the meganuclease. So, for example, the reason he achieved only 50% latent virus removal in one of the ganglions, was because the AAV he used in that experiment, did not transport the meganuclease to all the neurons of that ganglion. So he'll try and find an AAV (or combination of AAVS) that are better at transporting the meganuclease to that ganglion.

He's done his work on mice and he's either already started on guinea pigs or will be starting this year. Guinea pigs are much closer to humans than mice so we'll see how it goes.

Edited March 7, 2020 by Cas9